NR APUB
AU Botto,L.; Masserini,M.; Cassetti,A.; Palestini,P.
TI Immunoseparation of Prion protein-enriched domains from other detergent-resistant membrane fractions, isolated from neuronal cells
QU FEBS Letters 2004 Jan 16; 557(1-3): 143-7
IA http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4B8B4TF-5&_user=10&_coverDate=01%2F16%2F2004&_rdoc=28&_fmt=full&_orig=browse&_srch=doc-info(%23toc%234938%232004%23994429998%23476531%23FLA%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=56&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=965f90e9750faa3621be69d1ed958ce8
PT journal article
AB The possibility of coexistence of different subtypes of membrane lipid rafts has been investigated in cerebellar granule cells, by submitting detergent-resistant membrane fractions to immunoprecipitation. Among the proteins and lipids present in detergent-resistant fractions, almost all Prion protein, GAP43 and PKC were present in the immunoprecipitate obtained with anti-GAP43 or anti-Prion protein antibody at 4 degrees C, together with a small fraction of cholesterol and sphingolipids, suggesting that they belong to a distinct subset of membranes. On the contrary, all Fyn and almost all MARCKS remained in the supernatant. Fluorescence microscopy experiments showed that Fyn and Prion protein were mostly not colocalized within a single neuron. Our results suggest that granule cells membranes contains different subtypes of detergent-resistant fractions, possibly deriving from different lipid rafts.
MH Animals; Cerebellum/cytology; Detergents; Fluorescent Antibody Technique; GAP-43 Protein/isolation & purification; Membrane Proteins/*isolation & purification; Microscopy, Fluorescence; Nerve Tissue Proteins/isolation & purification; Neurons/*chemistry/cytology; Prions/*isolation & purification; Protein Kinase C/isolation & purification; Proteins/isolation & purification; Rats; Rats, Sprague-Dawley; Support, Non-U.S. Gov't
AD Laura Botto, Massimo Masserini, Paola Palestini, Department of Experimental, Environmental Medicine and Biotechnologies (DIMESAB), Medical School, University of Milano-Bicocca, Via Cadore 48, 20052 Monza, Italy; Arianna Cassetti, Consorzio MIA, Department of Neuroscience, University of Milano-Bicocca, Via Cadore 48, 20052, Monza, Italy
SP englisch
PO Niederlande