NR APKF
AU Nakamura,Y.; Sakudo,A.; Saeki,K.; Kaneko,T.; Matsumoto,Y.; Toniolo,A.; Itohara,S.; Onodera,T.
TI Transfection of prion protein gene suppresses coxsackievirus B3 replication in prion protein gene-deficient cells
QU Journal of General Virology 2003 Dec; 84(12): 3495-502
PT journal article
AB The susceptibility of prion protein gene (Prnp)-null cells to coxsackievirus B3 (CVB3) was investigated. Primary cultures of murine Prnp(-/-) brain cells were more sensitive to CVBs than corresponding cells from wild-type mice. The viral susceptibility of a Prnp-null cell line (HpL3-4) derived from the murine hippocampus was compared with that of two established cell lines (HeLa and HEp-2) that are widely employed for CVB3 studies. After infection with CVB3, HpL3-4 cells showed a very rapid and complete cytopathic effect (CPE). CPE developed earlier and viruses replicated at higher titres in HpL3-4 cells compared with HeLa and HEp-2 cells. Under a semi-solid medium, plaques developed rapidly in CVB3-infected HpL3-4 cells. To confirm the effect of Prnp on virus infection, a Prnp(-/-) cell line and a Prnp-transfected neuronal cell line were analysed. The replication and release of infectious particles of CVB3 in Prnp(-/-) cells were significantly more effective than those of the Prnp-transfected cell line. Levels of type I interferon (IFN) after CVB3 infection were higher in the Prnp-transfected cell line than in Prnp(-/-) cells, whereas apoptotic cells were more obvious in the Prnp(-/-) cells than in those of the Prnp-transfected cell line. These findings suggest that the absence of Prnp retards the induction of CVB3-induced IFNs, resulting in an enhanced CVB3 production and apoptotic cell death. Furthermore, our data indicate that the HpL3-4 cell line may provide a novel and sensitive system for isolation of CVB3 from clinical specimens.
MH Animals; Animals, Newborn; Apoptosis; Brain; Cells, Cultured; Comparative Study; Cytopathogenic Effect, Viral; Disease Models, Animal; Disease Susceptibility; Enterovirus B, Human/*physiology; Enterovirus Infections/prevention & control/*virology; Human; Interferon Type I/biosynthesis; Mice; Mice, Inbred C57BL; Neurons/metabolism/virology; Prions/biosynthesis/*genetics; Support, Non-U.S. Gov't; Transfection; Virus Replication
AD Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. Department of Clinical and Biological Sciences, University of Insubria, Varese, Italy. Laboratory for Behavioural Genetics, Brain Science Institute, RIKEN, Saitama, Japan.
SP englisch
PO England