NR APER
AU Arbel,M.; Lavie,V.; Solomon,B.
TI Generation of antibodies against prion protein in wild-type mice via helix 1 peptide immunization
QU Journal of Neuroimmunology 2003 Nov; 144(1-2): 38-45
PT journal article
AB We present here the development of antibodies against prion protein in BALB/C mice using as antigen human helix 1 of PrP. This sequence is suggested to be involved in protein pathological conformational changes, and is distinguished from that of mice by one amino acid. The immune tolerance to an 'almost-self' epitope and the poor immunogenicity of short peptides was overcome by using Multiple Antigen Peptide displaying eight copies of helix 1. The generated antibodies recognize the whole prion protein with a high binding constant and the established protocol may lead to an active immunization towards therapeutics of prion disease.
MH Animals; Antibodies, Heterophile/*biosynthesis/metabolism; Antibodies, Monoclonal/metabolism; Antigens, Heterophile/immunology/metabolism; Binding Sites, Antibody; Cattle; Cell Line, Tumor; Epitopes/immunology/metabolism; Human; Immune Sera/metabolism; Immunohistochemistry; Mice; Mice, Inbred BALB C; Peptide Fragments/*administration & dosage/*immunology/metabolism; PrPc Proteins/*immunology/metabolism; Protein Structure, Secondary; Rabbits; Sheep; Support, Non-U.S. Gov't; Vaccination/methods
AD Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
SP englisch
PO Niederlande