NR AOZP
AU Stuermer,C.A.O.; Wiechers,M.; Legler,D.; Hannbeck,S.; Plattner,H.
TI Capping of PrPc and co-capping with reggie microdomains in Jurkat T cells
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-22
PT Konferenz-Poster
AB
Reggie-1 and reggie-2 on the cytoplasmic face of the plasma membrane form distinct lipid raft signalling centers mediating the co-assembly of specific GPI-anchored surface proteins and intracellular effectors of transmembrane signalling (Stuermer et al., 2001; Rajendran et al., 2003).
To determine if PrPc associates with reggie microdomains and effects the co-assembly of molecular components involved in signalling, we employed antibody (AB) cross-linking of PrPc on Jurkat T cells. T lymphocytes are known to cluster molecules involved in T cell signalling in one aspect of the cell, the cap.
We demonstrate here that PrPc cross-linking induced capping in Jurkat T cells and co-capping with reggie-1 and -2. This is shown by double immunostainings and analysis at the light and electron microscope (EM) level, as well as by co-immunoprecipitation assays. Moreover, PrPc-reggie co-capping induced an increase of fyn, lck, phosphotyrosine and F-actin immunoreactivity in the cap region and led to the recruitment to the cap of the transmembrane T cell receptor/CD3 protein, as well as of Thy-1 and GM1, none of which was directly activated by their specific ABs.
Thus, PrPc co-capping with reggies activates pathways that can lead to T cell activation.
Supported by the "TSE Forschungsprogramm Baden-Württemberg".
AD C.A.O. Stuermer, M. Wiechers, D. Legler, S. Hannbeck, H. Plattner, University of Konstanz, Germany
SP englisch
PO Deutschland