NR AOYR
AU Sander,P.; Brenig,B.; Ziegler,U.; Groschup,M.H.; Leeb,T.
TI Analysis of Sequence Variability of the Bovine PRNP Gene in German Cattle Breeds
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-05
PT Konferenz-Poster
AB
Different alleles in the PRNP gene of man and sheep are known to be associated with varying susceptibilities to prion diseases. However, to date no polymorphisms in the protein coding region of the bovine PRNP gene with an effect on susceptibility to prion diseases have been identified. This study is dedicated to the detection of such polymorphisms in German breeds, which have been rarely studied in this regard. Our study includes eight animals each of Black-and-White German Holstein, Red-and-White German Holstein, Original German Holstein, German Yellow, German Simmental, four animals each of German Red Hill and of the Angler breed as well as seven BSE infected cattle of various breeds. In contrast to previous studies all three exons and the promoter region were investigated.
To date we identified 27 novel single nucleotide polymorphisms (SNPs), four single base insertion/deletion polymorphisms, and one 23 bp insertion/deletion polymorphism within the 5'-region of the PRNP gene. Additionally, we found two novel polymorphisms within the third exon, which had not been described so far.
In previous studies, the so called octapeptide repeat polymorphism in exon three was studied extensively in numerous cattle breeds. Among our samples we encountered five animals (9%) with a heterozygous (5/6) genotype and 50 (91%) animals with a (6/6) genotype. We did not find animals with the seven-octarepeat allele.
Our results confirm the high variability of the bovine PRNP gene that has been published in several studies on different cattle breeds. Sequence variants in the bovine PRNP gene could have an impact on expression level of the prion protein and thus on susceptibility to BSE. In this regard, the observed 23 bp insertion/deletion polymorphism in the 5' flanking region of the PRNP gene could be involved in differential PRNP expression.
AD P. Sander, T. Leeb, School of Veterinary Medicine Hannover, Germany; B. Brenig, University of Göttingen, Germany; U. Ziegler, M.H. Groschup, Federal Research Centre for Virus Diseases of Animals, Germany
SP englisch
PO Deutschland