NR AOYQ
AU Sandberg,M.K.; Löw,P.; Taraboulos,A.; Kristensson,K.
TI Expression of Presynaptic Proteins in Scrapie-infected GT1-1 Cells
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-94
PT Konferenz-Poster
AB
Prions are transmissible pathogens that cause neurodegenerative diseases but the mechanisms involved in the nervous system dysfunctions are unclear. To investigate neuronal dysfunction during prion infection, we use GT1-1 cells (neuronal cell line derived from mouse hypothalamus) infected with the scrapie RML strain. We have earlier described a disturbance in voltage-dependent N-type calcium channels in prion-infected GT1-1 (ScGT1-1) cells. Since different proteins in the SNARE complex partly can regulate the activity of voltage-dependent N-type calcium channels, the expression of presynaptic proteins was investigated in ScGT1-1 cells and control GT1-1 cells by western blot and immunohistochemistry. The most distinguished finding was an increase in the protein level of SNAP-25 in ScGT1-1 cells. The difference in expression of SNAP-25 in ScGT1-1 and uninfected GT1-1 cells was also determined following treatment with db-cAMP / IBMX that induce differentiation of these cells. These results indicate changes in the expression of presynaptic proteins that might directly, or indirectly, contribute to the disturbances of N-type calcium channels in prion-infected GT1-1 cells.
This research was supported by EC grant QLK2-CT-2002-81628
AD M.K. Sandberg, P. Löw, K. Kristensson, Dept. of Neuroscience, Karolinska Institutet, Stockholm, Sweden; A. Taraboulos, Dept. of Mol. Biology, Hadassah Medical School, Jerusalem, Israel
SP englisch
PO Deutschland