NR AOYK
AU Roth,K.; Zheng Xin Yan; Mauz,P.S.; Zenner,H.P.; Heeg,P.; Stitz,L.
TI New Requirements for Cleaning of Surgical Instruments due to vCJD
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-71
PT Konferenz-Poster
AB
Infectivity of pathological p A number of studies have shown that prion proteins resist many conventional chemical and physical procedures used in hospitals for the cleaning, disinfection or sterilization of surgical instruments. Currently there is concern among clinicians and health care professionals especially those involved in surgical procedures about the possibility of transmission of prion disease by surgical procedures.
We report an infectivity model using hamsters and steel wires to show transmission and decontamination of infectious prion proteins in brain materials. We show, that the hamster adapted pathological prion proteins tightly bind to steel wire and cause disease after the contaminated wires are implanted or even inserted for a very short time in the brain of healthy hamsters. When the contaminated wires were treated with different procedures before implantation infectivity was reduced which was manifested directly by prolonged survival time of the test animal. These results show, that this model can be used as a bioassay to validate reprocessing procedures for surgical instruments.
Procedures:
Enzymatic cleaning alone or followed by disinfection, autoclaving at 134 C, 18 min, or gas-plasma sterilisation.
Alkaline cleaning at 70 C alone or followed by disinfection, autoclaving at 134 C, 18 min, or gas-plasma sterilisation.
Cleaning with 1M NaOH, 24 h followed by autoclaving at 134 C, 18 min
Sterilisation with gas-plasma
Autoclaving 134 C, 18 min
Submerging in 59% H2O2
All positive controls died after 81 days. The brain of the hamsters was checked in western blot to prove that the death is related to TSE.
Only the group of hamsters, incubated by wires cleaned with alkaline detergents have a high surviving rate, independent if the cleaning is followed by disinfection or sterilization or not.
AD Klaus Roth, Zheng Xin Yan, SMP GmbH Pruefen Validieren Forschen; Tuebingen; Paul-Stefan Mauz, Hans Peter Zenner, Peter Heeg, Klinik der Eberhard-Karls Universität; Tuebingen; Lothar Stitz, Bundesforschungsanstalt für Viruskrankheiten von Tieren; Tuebingen
SP englisch
PO Deutschland