NR AOXW
AU Pichner,R.; Scherbel,C.; Müller,S.; Scherer,S.; Groschup,M.H.; Dietrich,R.; Märtlbauer,E.; Gareis,M.
TI Microbial inactivation of TSE-prions by the complex microflora of the gastrointestinal tract
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-96
PT Konferenz-Poster
AB
The fate of pathological forms of prions is insufficiently documented yet. Especially the degradation/inactivation by microorganisms has not been studied so far.
The aim of this study (part of the project: "Occurrence and stability of BSE agent in foodstuff and in the environment") was to find out whether the rumen or the intestinal flora is able to degrade or inactivate PrPsc. For this, samples were taken from contents of rumen and Colon ascendens of healthy adult cattle immediately after slaughter. Samples were incubated with homogenized brains of scrapie (strain 263K) infected hamsters up to 20 hours at 37 C both under anaerobic and aerobic conditions. A clearly visible decrease of the PrPsc signal (below the detection limit) could be observed after western blotting on X-ray films in all of the experiments. These results indicate for the first time the high ability of the microorganisms of the gastrointestinal tract to inactivate/degrade PrPsc.
IN
Die Autoren entnahmen gesunden, frisch geschlachteten Rindern Proben aus Pansen und Grimmdarm (Colon ascendens) und inkubierten diese bis zu 20 Stunden lang bei 37° mit Hirnhomogenaten von Hamstern, welche mit dem Hamster-Scrapie-Stamm 263K infiziert waren. Offensichtlich aufgrund enzymatischer Aktivität aus dem Magen-Darmtrakt der Rinder nahm die PrPsc-Konzentration in allen Experimenten derart stark ab, dass es im Western blot nicht mehr nachweisbar war.
Diese Arbeit erklärt, warum Kälber ein um mindestens eine Größenordnung höheres Infektionsrisiko als erwachsene Rinder haben [ANDI].
AD R. Pichner, C. Scherbel, M. Gareis, Institute for Microbiology and Toxicology, Federal Centre of Meat Research (BAFF), Kulmbach, Germany; S. Mueller, S. Scherer, Institute for Microbiology, ZIEL, Weihenstephan, Freising, Germany; M. Groschup, Institute for Novel and Emerging Diseases, Federal Research Centre for Viral Diseases of Animals, Riems, Germany; R. Dietrich, E. Maertlbauer, Institute for Hygiene and Technology of Milk, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany
SP englisch
PO Deutschland
ZF kritische Zusammenfassung von Roland Heynkes