NR AOXL
AU Nordström,E.K.; Luhr,K.M.; Löw,P.; Taraboulos,A.; Kristensson,K.
TI Rhythmic expression of prion protein, and PrP-knockdown in GT1 neuronal cells
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-92
PT Konferenz-Poster
AB
The normal prion protein (PrPc) has been suggested to be involved in regulation of circadian rhythms, since PrPc -/- mice show a disruption of both circadian activity rhythms and sleep patterns. Here we have used a cell culture system to study this hypothesis. Many clock-related transcripts have a circadian expression not only in the suprachiasmatic nucleus of the anterior hypothalamus, but also in other tissues. The treatment of certain cultured cells with high concentrations of serum induces a circadian expression of various genes that also oscillate in vivo. To investigate if PrPc and its homologue Doppel (Dpl) have circadian expression profiles in serum-treated GT1-7 neuronal cells, total RNA was isolated every 4th hour for 48 hours. RT-PCR analysis revealed that PrPc as well as Dpl oscillate with distinct 24 hour rhythms. We have also used RNA interference to knockdown PrPc expression in GT1-1 cells, which enables studies on the effect of PrPc on the expression profile of Dpl and various clock-related transcripts. Recent studies suggest a circadian expression of PrPc in the rat brain and GT1-7 cells may serve as a useful model system to investigate the mechanisms by which PrPc is involved in regulation of circadian rhythms.
This research was supported by EC grant QLK2-CT-2002-81628.
AD E.K. Nordström, K.M. Luhr, P. Löw, K. Kristensson, Department of Neuroscience, Karolinska Institutet, Sweden; A. Taraboulos, Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Israel
SP englisch
PO Deutschland