NR AOWQ
AU McLennan,N.F.; Brennan,P.M.; McNeill,A.; Davies,I.; Fotheringham,A.; Head,M.W.; Graham,D.; Ironside,J.W.; Williams,A.; Bell,J.E.
TI Prion protein accumulation and neuroprotection in hypoxic injury
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-13
PT Konferenz-Poster
AB The function of the prion protein, PrPc, remains unclear although several lines of research have suggested a role in the cellular response to oxidative stress. Here we investigate the expression of PrPc in hypoxic brain tissues to examine the proposal that PrPc can be induced by neuronal stress. Tissues from cases of adult cerebral ischaemia and perinatal hypoxic-ischaemic injury were compared with control tissues for the detection and expression of PrPc. PrPc immunoreactivity accumulated within the penumbra of white matter hypoxic damage within adult brain, and within neuronal soma and microglia in cases of perinatal hypoxic-ischaemic injury. In situ hybridization analysis suggested a transcriptional up-regulation of PrP mRNA during hypoxia. Analysis of brain tissue from an experimental mouse stroke-model, permanent middle cerebral artery occlusion, also demonstrated an accumulation of PrPc in neuronal soma within the penumbra of ischaemic lesions. Furthermore, the infarct size in PrP-null mice was significantly greater than in the wild-type in this model. These data support the proposed role for PrPc in the neuroprotective adaptive cellular response to hypoxic injury.
AD N.F. McLennan, P.M. Brennan, A. McNeill, M.W. Head, J.W. Ironside, J.E. Bell, University of Edinburgh, UK; I. Davies, A. Fotheringham, University of Manchester, UK; D. Graham, A. Williams, University of Glasgow, UK
SP englisch
PO Deutschland