NR AOVX
AU Lipp,O.; Ritzmann,M.; Heinritzi,K.; Truyen,U.
TI Study on the genetic variability of the porcine prion protein gene in German pig breeds
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-08
PT Konferenz-Poster
AB
The genetic variability of the prion protein gene may influence the susceptibility of the host to infection with TSE agents. This has been shown for mice and sheep and is strongly suggested for man.
Pigs have been shown to be susceptible to the BSE agent after intracerebral, but not after oral inoculation. In these studies, however, only a very limited number of pigs of a restricted genetic background were used. Therefore the question arose whether those experiments do reflect the susceptibility of the species pig or rather that of a single resistant pig breed.
We therefore analysed the genetic variability of various German pig breeds.
The prion protein gene of a total of 53 pigs from 10 different breeds (2 x Deutsche Landrasse, 3 x Deutsches Edelschwein, 4 x Leicoma, 4 x Duroc, 11 x Pietrain, 6 x Large White, 9 x Hampshire, 2 x Belgische Landrasse, 8 x Schwäbisch Hällisches Schwein, 4 x Wildschwein (wild boar)) was amplified by PCR and directly sequenced. The sequences obtained were aligned to the published sequence (Martin et al., 1995).
All sequences were found to be identical and identical to the published sequence.
Our findings strongly suggest that there is not a marked heterogeneity of the PrP gene in pigs as seen for sheep or mice. The results of limited inoculation experiments in pigs that were based only a single pig breed may therefore be applied to the species pig, and the results obtained in those studies may therefore be of general importance.
Martin et al., Direct sequencing of PCR amplified pig PrP genes. Biochem. Biophys. Acta 1270, 211-214.
AD Ortrun Lipp, Tiergesundheitsdienst Bayern e.V., Poing, Germany; Mathias Ritzmann, Karl Heinritzi, II. Medizinische Tierklinik, Ludwig-Maximilians-Universität München, Germany; Uwe Truyen, Institut für Tierhygiene und Öffentliches Veterinärwesen, Universität Leipzig, Germany
SP englisch
PO Deutschland