NR AOVM
AU Lasch,P.; Schmitt,J.; Beekes,M.; Udelhoven,T.; Eiden,M.; Fabian,H.; Petrich,W.; Naumann,D.
TI Ante mortem identification of BSE from serum by infrared spectroscopy
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-16
PT Konferenz-Poster
AB
Here we report on a novel method for the ante mortem diagnosis of BSE in cattle. The technique is based on infrared spectroscopy of bovine serum samples and advanced multivariate pattern methodologies. Based on serum measurements of more than 1100 animals IR spectroscopy consistently revealed characteristic structural and compositional alterations in the sera of BSE infected animals. These BSE specific spectral changes were utilized to establish classification models for the diagnosis of BSE. In order to test whether the new IR methodology is able to discriminate between BSE positive and control animals, the classification models were validated with spectra from animals with previously unknown (blinded) diagnosis for BSE. The group of control animals contained also cattle suffering from classical viral or bacterial infections
Within the scope of two field studies infrared spectra from 1136 animals were recorded. Artificial neural network classifiers and classifiers based on linear discriminant analysis were trained with spectra from 701 (study I) or 843 (study II) animals. The classifiers were then utilized to establish 'spectral diagnoses' for 92 (validation study I) or 293 (validation study II) animals with unknown status of BSE. After unblinding the diagnoses, sensitivities of 85% and 84% and specificities of 86% and 91% were determined, respectively. The combination of different classification algorithms increased the sensitivity and specificity of BSE diagnosis to 96% and 92%, respectively.
Our findings suggest that further development of the infrared methodology could lead towards an automatic, objective and fast analytical tool for the ante mortem diagnosis of BSE.
1. Schmitt, J.; Beekes, M.; Brauer, A.; Udelhoven, T.; Lasch, P.; Naumann, D. Identification of Scrapie Infection from Blood Serum by Fourier Transform Infrared Spectroscopy. Anal Chem. 2002. 74, 3865-3868.
2. Lasch, P.; Schmitt, J.; Beekes, M.; Udelhoven, T.; Eiden, M.; Fabian, H.; Petrich, W.; Naumann, D. Ante mortem identification of BSE from serum using infrared spectroscopy. submitted.
AD Peter Lasch, Michael Beekes, Heinz Fabian, Dieter Naumann, Robert Koch Institut, P13 & P26, Berlin, Germany; Jürgen Schmitt, Thomas Udelhoven, Michael Eiden, Synthon KG, Heidelberg, Germany; Wolfgang Petrich, Roche Diagnostics, Mannheim, Germany
SP englisch
PO Deutschland