NR AOTQ
AU Hanlon,J.; Sacchi,C.; Doyle,D.; Dolan,S.; Monks,E.; Weavers,E.D.; Rogers,M.
TI Evaluation of non-PrP markers of BSE
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-55
PT Konferenz-Poster
AB
Protein expression profiles within cells are altered in response to internal and external stimuli. We have attempted to exploit altered expression of proteins in BSE infected cases to identify a diagnostic marker for BSE in samples accessible from the live animal.
A number of proteins or mRNAs have been identified over the last ten years that exhibit altered expression levels in TSE infections. In this study we were looking for a marker with a high sensitivity and specificity which was present in a body fluid which is easily accessible in the live animal.
The following nine proteins were investigated: Apolipoprotein E , beta-2-microglobulin, a-crystallin, cathepsin D, metallothionein, MHC classI, transferrin, Apolipoprotein J and ubiquitin.
The concentration of these proteins in normal and BSE infected bovine brain was assessed by western blotting and immunohistochemical techniques, to confirm that their expression is altered during BSE infection as in other TSE infections. Of the nine proteins investigated only MT was found to be altered in BSE positive brains. A marked increase in MT immunoreactivity was found throughout grey matter , particularly in areas of PrPBSE deposition and vacuolar change.
Plasma, CSF and urine samples from normal and BSE animals were subsequently analysed as potential target samples for a live test. There is some evidence of altered expression in CSF samples with and a- B-crystallin and Cathepsin D. None of the proteins analysed showed any difference between negative and positive samples in either plasma or urine samples as tested.
AD J. Hanlon, C. Sacchi, D. Doyle, S. Dolan, M. Rogers, Department of Zoology and Conway Institute of Biomolecular and Biomedical Sciences, UCD, Ireland; E. Monks, E. Weavers, Veterinary Research Laboratory, Ireland
SP englisch
PO Deutschland