NR AOSV
AU Galand,S.; Truchot,L.; Meyronet,D.; Gros,E.; Kopp,N.; Perret-Liaudet,A.; Streichenberger,N.
TI GABAergic neuronal subpopulations in 24 putamina of Creutzfeldt-Jakob disease: identification and quantification of specific neuronal loss.
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-88
PT Konferenz-Poster
AB
Spongiform change, neuronal loss and astrocytic gliosis are histological characteristic features of Creutzfeldt-Jakob disease (CJD). These lesions are probably linked to the codon 129 genotype and to the pathological prion protein (PrPsc) type (1).
The GABAergic neuronal system, including neurons immunoreactive for calcium binding proteins (calbindin D28K, calretinin and parvalbumin), seems to be a preferential target in CJD (2). Isolectin B4 is an extracellular matrix marker used to stain perineuronal nets surrounding parvalbumin inhibitory interneurons subpopulation. These neurons are early affected in CJD (3).
The putamen is regularly impaired in CJD and is mainly filled with GABAergic neurons.
The aim of this study was to characterize GABAergic subpopulations affected in putamen of CJD and to quantify neuronal loss as well as astrocytic gliosis.
In this work, immunohistochemistry was performed on putamen of 24 CJD and 14 control cases. Immunoreactive cells were quantified by semi automatic method. Neuronal loss and astrocytic gliosis data were compared to spongiform change quantification previously realized on the same CJD cases (4). The three histological criteria were correlated to codon 129 genotype and PrPsc type.
1 Parchi P et al. Classification of sporadic Creutzfeldt-Jakob disease based on molecular and phenotypic analysis of 300 patients. Ann Neurol. 1999; 46:224-233.
2 Guentchev M et al. Severe, early and selective loss of a subpopulation of GABAergic inhibitory neurons in experimental transmissible spongiform encephalopathies. Brain Pathol. 1998, 8(4):615-23.
3 Belichenko P et al. Early destruction of the extracellular matrix around parvalbumin-immunoreactive interneurons in Creutzfeldt-Jakob disease. Neurobiol Dis. 1999, 6(4):269-79.
4 Truchot L et al. Quantification of spongiform change in putamen of 30 control cases versus 30 CJD cases using a semi automatic method. International conference for transmissible spongiform encephalopathies, Edinburgh 2002.
AD S. Galand, L. Truchot, E. Gros, N. Kopp, A. Perret-Liaudet, N. Streichenberger, Hopital Neurologique, Lyon France; D. Meyronet, Inserm U433, Lyon France
SP englisch
PO Deutschland