NR AOQX
AU Buschmann,A.; Biacabe,A.G.; Gretzschel,A.; Bencsik,A.A.; Madec,J.Y.; Ziegler,U.; Erhardt,G.; Lühken,G.; Baron,T.G.M.; Groschup,M.H.
TI Two biochemical phenotypes in German and French ovine and caprine scrapie cases
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-59
PT Konferenz-Poster
AB Since the introduction of an intensified scrapie epidemiosurveillance in 2002, the numbers of notified scrapie cases have increased dramatically throughout the European Union. The testing is being performed using one of the three available BSE rapid tests that have passed the first EU evaluation. These tests include an indirect and a sandwich ELISA as well as a Western blot. Following a reactive result in one of the rapid tests, such samples have to be retested in the national TSE reference laboratory using one of the confirmatory methods which are either a preparation of the scrapie-associated fibrils (SAF) and subsequent Western blot, or an immunohistochemical staining (IHC). In retrospective examinations among the 43 German and 186 French (171 sheep and 15 goats) scrapie cases, it became obvious that some of the samples (20 in France and 21 in Germany) could not be detected using two of the available rapid tests. As there are some differences in the rapid test sample preparation protocols, we replaced single buffers and reagents from the preparation protocol of the rapid test Western blot with other buffers. We were then able to confirm the positive result from the confirmatory testing for most samples. We suggest that these non-uniform reaction patterns are determined by differences in the antigenicity and physicochemical characteristics of the pathological prion protein that is used as a diagnostic marker in all tests. Interestingly, this reaction pattern does not seem to be directly linked to one specific allele of the ovine prion protein. Moreover, the pathological prion protein of none of these phenotypically different samples shows biochemical properties that may indicate a BSE infection in these animals. Similar divergent results have never been observed during rapid testing of bovine samples for BSE.
AD Anne Buschmann, Anja Gretzschel, Ute Ziegler, Martin H. Groschup, Friedrich-Loeffler-Institut (FLI), Federal Research Centre for Virus Diseases of Animals, Institute for Novel and Emerging Infectious Diseases, Boddenblick 5a, 17493 Greifswald-Insel Riems, Germany; Anne-Gaelle Biacabé, Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Jean-Yves Madec, Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Georg Erhardt, Gesine Lühken (gesine.luehken@agrar.uni-giessen.de), Department of Animal Breeding and Genetics, Justus-Liebig University of Giessen, 35390 Giessen, Germany
SP englisch
PO Deutschland