NR AOPW
AU Baier,M.; Norley,S.; Schultz,J.; Burwinkel,M.; Schwarz,A.; Riemer,C.
TI Prion diseases: infectious and lethal doses following oral challenge
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-52
PT Konferenz-Poster
AB A brain homogenate prepared a terminally ill hamster infected with scrapie strain 263K was serially diluted and administered orally to groups of hamsters. The undiluted brain homogenate led to clinical scrapie in all animals inoculated. The attack rate decreased for dilutions of the homogenate and subclinical infections were identified among the healthy survivors at 520 dpi by Western-blotting. The number of animals succumbing to disease and the combined number of Western-blot positive survivors plus diseased hamsters were used to calculate the 50% lethal dose (LD50) and 50% infectious dose (ID50) of the inoculum. The model system represents an approximation to the transmission of TSEs such as vCJD via dietary exposure to the infectious agent and suggests that, due to the rather small difference between the calculated LD50 and ID50, the number of clinical cases will not be vastly exceeded by the number of subclinical carriers of the disease.
AD M. Baier, S. Norley, J. Schultz, M. Burwinkel, A. Schwarz, C. Riemer, Neurodegenerative Diseases, Robert-Koch-Institute, Berlin, Germany
SP englisch
PO Deutschland