NR AOGN
AU Kubosaki,A.; Nishimura-Nasu,Y.; Nishimura,T.; Yusa,S.; Sakudo,A.; Saeki,K.; Matsumoto,Y.; Itohara,S.; Onodera,T.
TI Expression of normal cellular prion protein (PrPc) on T lymphocytes and the effect of copper ion: Analysis by wild-type and prion protein gene-deficient mice.
QU Biochemical and Biophysical Research Communications 2003 Aug 8; 307(4): 810-3
PT journal article
AB The purpose of this report was to determine the effect of prion protein (PrP) gene disruption on T lymphocyte function. Previous studies have suggested that normal cellular prion protein (PrPc) binds to copper and Cu(2+) is essential for interleukin-2 (IL-2) mRNA synthesis. In this study, IL-2 mRNA levels in a copper-deficient condition were investigated using T lymphocytes from prion protein gene-deficient (PrP(0/0)) and wild-type mice. Results showed that Cu(2+) deficiency had no effect on PrPc expression in Con A-activated splenocytes. However, a delay in IL-2 gene expression was observed in PrP(0/0) mouse T lymphocyte cultures using Con A and Cu(2+)-chelator. These results suggest that PrPc expression may play an important role in rapid Cu(2+) transfer in T lymphocytes. The rapid transfer of Cu(2+) in murine T lymphocytes could be one of the normal functions of PrPc.
MH Amino Acid Sequence; Animal; Cells, Cultured; Copper/metabolism/*pharmacology; Interleukin-2/biosynthesis/genetics; Mice; Mice, Knockout; Molecular Sequence Data; PrPc Proteins/genetics/metabolism/*physiology; RNA, Messenger/biosynthesis; Support, Non-U.S. Gov't; T-Lymphocytes/drug effects/*immunology/metabolism
AD Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
SP englisch
PO USA