NR ANYK
AU Lezmi,S.; Bencsik,A.A.; Monks,E.; Petit,T.; Baron,T.G.M.
TI First case of feline spongiform encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland.
QU Histochemistry and Cell Biology 2003 May; 119(5): 415-22
PT case reports; journal article
AB Feline spongiform encephalopathy (FSE), affecting domestic and captive feline species, is a prion disease considered to be related to bovine spongiform encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
IN Lezmi et al. konnten beim ersten in Frankreich entdeckten Gepard mit FSE immunhistochemisch PrPsc in Hirnrinde (Cerebral cortex), Hirnstamm (Brain stem), Kleinhirn (Cerebellum), Rückenmark im Halswirbelbereich (Cervical spinal cord), Rückenmark im Brustwirbelbereich (Thoracic spinal cord), Rückenmark im Lendenwirbelbereich (Lumbar spinal cord), Netzhaut (Retina) als Teilen des Zentralnervensystems nachweisen. Sie wiesen so aber auch PrPsc im zwischen Halswirbelsäule und Schultergelenk liegenden Nervengeflecht Plexus brachialis (Brachial plexus), in den Lymphknoten des Gekröses (Mesenteric lymph node), außen auf dem Unterkiefer (Mandibular lymph node) und der Kniebeuge (Popliteal lymph node) sowie in Niere (Kidney) und Nebenniere (Adrenal gland) nach. Kein PrPsc fanden die Autoren in den Nervensystemen um Speiseröhre (Oesophagus), Magen (Stomach), Dünndarm (Small intestine) und Dickdarm (Large intestine), in Milz (Spleen), Blase (Bladder), Leber (Liver), Nasenschleimhaut (Nasal mucosa), Eierstock (Ovary) Gebärmutter (Uterus), Bauchspeicheldrüse (Pancreas), Speicheldrüse (Salivary gland), gestreiftem Muskel (Striated muscle) und Schilddrüse (Thyroid gland).
MH Adrenal Glands/metabolism/*pathology; Animals; *Animals, Zoo; Central Nervous System/metabolism/pathology; *Cheetahs; Female; France; Immunoenzyme Techniques/veterinary; Kidney Glomerulus/metabolism/*pathology; Lymph Nodes/metabolism/pathology; Peripheral Nervous System/metabolism/pathology; PrPsc Proteins/*metabolism; Prion Diseases/metabolism/pathology/*veterinary; Support, Non-U.S. Gov't; Zona Fasciculata/metabolism/pathology; Zona Reticularis/metabolism/pathology
AD Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Eoin Monks, Department of Agriculture, Food and Rural Development, Central Veterinary Research Laboratory, Abbotstown, Castleknock, Dublin, Ireland; Thierry Petit, Zoo de la Palmyre, 17570 Les Mathes, BP08, France
SP englisch
PO Deutschland