NR ANVB
AU Rachidi,W.; Mange,A.; Senator,A.; Guiraud,P.; Riondel,J.; Benboubetra,M.; Favier,A.; Lehmann,S.
TI Prion infection impairs copper binding of cultured cells
QU The Journal of Biological Chemistry 2003 Apr 25; 278(17): 14595-8
IA http://www.jbc.org/cgi/content/full/278/17/14595
PT journal article
AB The molecular mechanism of neurodegeneration in transmissible spongiform encephalopathies (TSEs) remains unclear. Using radioactive copper ((64)Cu) at physiological concentration, we showed that prion infected cells display a marked reduction in copper binding. The level of full-length prion protein known to bind the metal ion was not modified in infected cells, but a fraction of this protein was not releasable from the membrane by phosphatidylinositol-specific phospholipase C. Our results suggest that prion infection modulates copper content at a cellular level and that modification of copper homeostasis plays a determinant role in the neuropathology of TSE.
MH Cells, Cultured; Copper/*metabolism; Copper Radioisotopes; Human; Kinetics; Neurodegenerative Diseases/etiology/metabolism; Neurons/*pathology; Phospholipase C/metabolism; Prion Diseases/etiology/*metabolism; Prions/metabolism; Support, Non-U.S. Gov't
AD Walid Rachidi, Abderrahmene Senator, Pascale Guiraud, Jacqueline Riondel, Laboratoire Biologie Stress Oxydant, Faculte de Pharmacie, Domaine de La Merci, 38706 La Tronche-Grenoble, France; Alain Mangé, Sylvain Lehmann, Institut de Genetique Humaine, CNRS U.P.R. 1142, 141, rue de la Cardonille, 34396 Montpellier, France; Mustapha Benboubetra, Laboratory of Applied Biochemistry, Faculty of Sciences, University of Setif, 19000 Setif, Algeria; Alain Favier, Laboratoire des Lesions des Acides Nucleiques, CNRS/Commissariat a l'Energie Atomique, 5046, Avenue des Martyrs, 38000 Grenoble, France
SP englisch
PO USA