NR ANQP
AU Heggebo,R.; Press,C.McL.; Gunnes,G.; Ulvund,M.J.; Tranulis,M.A.; Landsverk,T.
TI Detection of PrPsc in Lymphoid Tissues of Lambs Experimentally Exposed to the Scrapie Agent
QU Journal of Comparative Pathology 2003 Feb-Apr; 128(2-3): 172-81
PT journal article
AB Histoblotting and immunohistochemistry were used to detect disease-associated prion protein (PrPsc) in lymphoid tissues of lambs of known PrP genotype infected with the scrapie agent by stomach tube at the age of 2 months. The ileal and jejunal Peyer's patches and retropharyngeal and distal jejunal lymph nodes were studied 1 week, 5 weeks, 5 months and 11 months after inoculation. Other lymphoid tissues examined included superficial cervical lymph node, tonsil and spleen. PrPsc was not detected in any tissue of any lamb at 1 week post-inoculation. At 5 weeks, PrPsc was detected in tissues of lambs of susceptible PrP genotypes (AV(136)QQ(171) and VV(136)QQ(171)), but not lambs of other PrP genotypes (AA(136)QQ(171), AA(136)QR(171) and AV(136)QR(171)). PrPsc was present in the germinal centres of tonsils, distal jejunal and retropharyngeal lymph nodes, and spleen. In the nodules of ileal and jejunal Peyer's patches, only occasional solitary cells showed the presence of PrPsc. At 5 months post-inoculation, increased accumulations of PrPsc were detected in ileal and jejunal Peyer's patches, as well as in the retropharyngeal and distal jejunal lymph nodes of a single lamb inoculated with the agent from a sheep of the same susceptible PrP genotype. Eleven months after exposure to the scrapie agent, PrPsc was detected in all lymphoid tissues examined from sheep of susceptible PrP genotypes. These studies show that PrPsc was detectable in lymphoid tissues 5 weeks after exposure to the scrapie agent by stomach tube in lambs as young as 3 months of age and indicate that the PrP genotype is a significant factor for the rapid uptake and spread of the agent through lymphoid tissues.
AD Department of Sheep and Goat Research, Norwegian School of Veterinary Science, Kyrkjevegen 332/334, N-4325, Sandnes, Norway
SP englisch
PO England
OR Prion-Krankheiten H