NR AMXN
AU Zanata,S.M.; Lopes,M.H.; Mercadante,A.F.; Hajj,G.N.M.; Chiarini,L.B.; Nomizo,R.; Freitas,A.R.O.; Cabral,A.L.B.; Lee,K.S.; Juliano,M.A.; de Oliveira,E.; Jachieri,S.G.; Burlingame,A.L.; Huang,L.; Linden,R.; Brentani,R.R.; Martins,V.R.
TI Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection
QU EMBO Journal 2002 Jul 1; 21(13): 3307-16
PT journal article
AB Prions are composed of an isoform of a normal sialoglycoprotein called PrPc, whose physiological role has been under investigation, with focus on the screening for ligands. Our group described a membrane 66 kDa PrPc-binding protein with the aid of antibodies against a peptide deduced by complementary hydropathy. Using these antibodies in western blots from two-dimensional protein gels followed by sequencing the specific spot, we have now identified the molecule as stress-inducible protein 1 (STI1). We show that this protein is also found at the cell membrane besides the cytoplasm. Both proteins interact in a specific and high affinity manner with a K(d) of 10(-7) M. The interaction sites were mapped to amino acids 113-128 from PrPc and 230-245 from STI1. Cell surface binding and pull-down experiments showed that recombinant PrPc binds to cellular STI1, and co-immunoprecipitation assays strongly suggest that both proteins are associated in vivo. Moreover, PrPc interaction with either STI1 or with the peptide we found that represents the binding domain in STI1 induce neuroprotective signals that rescue cells from apoptosis.
MH Animal; Anisomycin/antagonists & inhibitors/pharmacology; *Apoptosis/drug effects; Binding Sites; Copper/metabolism; Cyclic AMP/physiology; Cyclic AMP-Dependent Protein Kinases/physiology; Eye Proteins/chemistry/metabolism; Heat-Shock Proteins/chemistry/isolation & purification/*metabolism; Hydrophobicity; Laminin/metabolism; Macromolecular Systems; Membrane Proteins/metabolism; Mice; Molecular Chaperones/chemistry/isolation & purification/*metabolism; Nerve Tissue Proteins/chemistry/isolation & purification/*metabolism; Neurons/cytology/*metabolism; Organ Culture; Peptide Fragments/metabolism; Phosphoprotein Phosphatase/chemistry/*metabolism; Protein Binding; Protein Interaction Mapping; Recombinant Fusion Proteins/metabolism; Retina/cytology/drug effects; Signal Transduction; Support, Non-U.S. Gov't
AD Ludwig Institute for Cancer Research, Sao Paulo Branch, Rua Prof. Antonio Prudente 109 4A, Sao Paulo 01509010, Brasil.
SP englisch
PO England