NR AMNH
AU Wiley,C.A.; Burrola,P.G.; Buchmeier,M.J.; Wooddell,M.K.; Barry,R.A.; Prusiner,S.B.; Lampert,P.W.
TI Immuno-gold localization of prion filaments in scrapie-infected hamster brains
QU Laboratory Investigation 1987 Dec; 57(6): 646-56
PT journal article
AB The brains of scrapie-infected hamsters have been examined for the presence of structures antigenically related to the prion protein (PrP 27-30). Glutaraldehyde-perfused hamster brains, 72 days postinfection, were immunostained using rabbit monospecific antisera raised against synthetic peptides corresponding to the N-terminal 13 or 15 amino acids of PrP 27-30, and using rabbit antisera raised against infectious prions or PrP 27-30 purified from scrapie-infected hamster brains. Antisera to the synthetic peptides stained extracellular filaments in agreement with previous immunoperoxidase studies which used affinity-purified PrP 27-30 antibodies; in addition to subependymal and subpial localization, we show ventricular and perivascular staining. Using a colloidal gold-secondary antibody technique, we have demonstrated that the antibodies labeled filaments measuring 7 to 17 nm in diameter. Whereas most of the periventricular and perivascular filaments appeared extracellular, some appeared to be within processes intimately associated with ependymal cells, degenerating membranes of astrocytes, and neurites.
MH Amino Acid Sequence; Animal; Antibody Specificity; Brain/*microbiology/ultrastructure; Brain Chemistry; Female; Hamsters; Immune Sera; Molecular Sequence Data; Nerve Tissue Proteins/*analysis/immunology; PrP 27-30 Protein; Prions/*analysis/immunology/ultrastructure; Scrapie/metabolism/*microbiology/pathology; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
AD Department of Pathology, University of California San Diego, La Jolla.
SP englisch
PO USA