NR AMFH
AU Wang,S.; Cockett,N.E.; Miller,J.M.; Shay,T.L.; Maciulis,A.; Sutton,D.L.; Foote,W.C.; Holyoak,G.R.; Evans,R.C.; Bunch,T.D.; Beever,J.E.; Call,J.W.; Taylor,W.D.; Marshall,M.R.
TI Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie
QU Theriogenology 2002 Apr 15; 57(7): 1865-75
PT journal article
AB The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA(136)QQ(171) (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep. Although embryos were collected from scrapie-positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.
MH Alleles; Animal; Disease Transmission, Vertical; Embryo Transfer/*veterinary; Female; Gene Frequency; Genotype; *Polymorphism (Genetics); Prions/*genetics; Scrapie/*genetics/prevention & control/transmission; Sheep; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.; Tissue Harvesting/methods/veterinary
AD Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-4815, USA
SP englisch
PO USA