NR AMEY
AU Walton,M.; Saura,J.; Young,D.; MacGibbon,G.; Hansen,W.; Lawlor,P.; Sirimanne,E.; Gluckman,P.; Dragunow,M.
TI CCAAT-enhancer binding protein alpha is expressed in activated microglial cells after brain injury
QU Brain Research. Molecular Brain Research 1998 Oct 30; 61(1-2): 11-22
PT journal article
AB Microglial cells play important roles in brain injury and repair and are implicated in diseases such as Alzheimer's disease, Creutzfeldt-Jakob disease, multiple sclerosis, the Aids Dementia Complex and stroke. Despite their importance in neuropathology, the underlying molecular basis for the activation of microglia after brain injury is not understood. We show, using RT-PCR, in situ hybridisation, immunocytochemistry, and electrophoretic mobility shift assay, that the CCAAT-enhancer binding protein alpha (C/EBP alpha), a sequence specific DNA-binding protein, is induced in microglial cells, but not astrocytes or neurons, after hypoxic-ischemic brain injury. These results suggest that C/EBP alpha might regulate gene expression and consequentially have a role in the activation and/or proliferation of microglia following brain injury.
MH Animal; Brain Chemistry; Brain Injuries/*genetics; CCAAT-Enhancer-Binding Proteins; DNA-Binding Proteins/*biosynthesis/physiology; *Enhancer Elements (Genetics); *Gene Expression Regulation; In Situ Hybridization; Male; Microglia/*metabolism/*pathology; Nuclear Proteins/*biosynthesis/physiology; RNA, Messenger/analysis; Rats; Rats, Wistar; Reperfusion Injury/genetics; Support, Non-U.S. Gov't; Transcription Factors
AD Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
SP englisch
PO Niederlande