NR AMCA
AU Volkov,K.V.; Aksenova,A.Y.; Soom,M.J.; Osipov,K.V.; Svitin,A.V.; Kurischko,C.; Shkundina,I.S.; Ter-Avanesyan,M.D.; Inge-Vechtomov,S.G.; Mironova,L.N.
TI Novel non-Mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae
QU Genetics 2002 Jan; 160(1): 25-36
PT journal article
AB Two cytoplasmically inherited determinants related by their manifestation to the control of translation accuracy were previously described in yeast. Cells carrying one of them, [PSI(+)], display a nonsense suppressor phenotype and contain a prion form of the Sup35 protein. Another element, [PIN(+)], determines the probability of de novo generation of [PSI(+)] and results from a prion form of several proteins, which can be functionally unrelated to Sup35p. Here we describe a novel nonchromosomal determinant related to the SUP35 gene. This determinant, designated [ISP(+)], was identified as an antisuppressor of certain sup35 mutations. We observed its loss upon growth on guanidine hydrochloride and subsequent spontaneous reappearance with high frequency. The reversible curability of [ISP(+)] resembles the behavior of yeast prions. However, in contrast to known prions, [ISP(+)] does not depend on the chaperone protein Hsp104. Though manifestation of both [ISP(+)] and [PSI(+)] is related to the SUP35 gene, the maintenance of [ISP(+)] does not depend on the prionogenic N-terminal domain of Sup35p and Sup35p is not aggregated in [ISP(+)] cells, thus ruling out the possibility that [ISP(+)] is a specific form of [PSI(+)]. We hypothesize that [ISP(+)] is a novel prion involved in the control of translation accuracy in yeast.
MH Alleles; Chromosomes, Fungal; Culture Media/metabolism; Extrachromosomal Inheritance; Fungal Proteins/*genetics; Gene Expression Regulation, Fungal; Genes, Dominant; Guanidine/metabolism; Heat-Shock Proteins/physiology; Prions/genetics; Saccharomyces cerevisiae/*genetics/metabolism; Support, Non-U.S. Gov't; Suppression, Genetic; Translation, Genetic/*physiology
AD Department of Genetics, St. Petersburg State University, St. Petersburg 199034, Russia.
SP englisch
PO USA