NR ALWQ
AU Vaiman,D.; Billault,A.; Tabet-Aoul,K.; Schibler,L.; Vilette,D.; Oustry-Vaiman,A.; Soravito,C.; Cribiu,E.P.
TI Construction and characterization of a sheep BAC library of three genome equivalents
QU Mammalian Genome 1999 Jun; 10(6): 585-7
PT journal article
AB A sheep BAC library of over three genome equivalents was constructed and arrayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was estimated at 123 kb. The library was screened by PCR with 77 primer pairs corresponding to ovine microsatellites distributed throughout the genome. The probability of finding a random sequence in the library could be estimated at 0.96.
MH Animal; *Chromosomes, Bacterial; Deoxyribonuclease HindIII/genetics; *Gene Library; Genome; In Situ Hybridization, Fluorescence; Male; Microsatellite Repeats; Prions/genetics; Sheep/*genetics; Support, Non-U.S. Gov't; X Chromosome
AD Daniel Vaiman, Laurent Schibler, Didier Vilette, Anne Oustry-Vaiman, Edmond P. Cribiu, Laboratoire de Genetique biochimique et de Cytogenetique, Departement de Genetique Animale, INRA, 78352 Jouy-en-Josas, France; Alain Billault, Catherine Soravito, Fondation Jean Dausset-Centre d'Etude du Polymorpisme Humain, 27, rue Juliette Dodu, 75010 Paris, France; Kamila Tabet-Aoul, 3Laboratoire de Pathologie infectieuse et d?Immunologie, INRA, 37380, Nouzilly, France
SP englisch
PO USA