NR ALRV
AU Toh,B.H.; Gibbs,C.J.Jr.; Gajdusek,D.C.; Goudsmit,J.; Dahl,D.
TI The 200- and 150-kDa neurofilament proteins react with IgG autoantibodies from patients with kuru, Creutzfeldt-Jakob disease, and other neurologic diseases
QU Proceedings of the National Academy of Sciences of the United States of America 1985 May; 82(10): 3485-9
PT journal article
AB Sera from 65 patients with spongiform virus encephalopathies (29 with kuru, 36 with Creutzfeldt-Jakob disease), 79 with other neurologic diseases, and 65 control subjects were examined for reactivity in immunoblots of preparations of myelinated axons and neurofilaments from mouse brain. The sera reacted most frequently with the 200-kDa and 150-kDa neurofilament proteins and less frequently with the 70-kDa neurofilament protein and a 62-kDa neurofilament-associated protein. The sera reacted with the same proteins as those which reacted with rabbit and mouse polyclonal antibodies and mouse monoclonal antibody to neurofilament proteins. Serum reactions were also seen with Trixon X-100 extracts of chimpanzee brain and bovine spinal cord but not with Triton extracts of liver, kidney, and muscle.
MH Alzheimer Disease/*immunology; Animal; Antibody Specificity; Autoantibodies/immunology; Brain/immunology; Creutzfeldt-Jakob Syndrome/*immunology; Cytoskeleton/*immunology; Epitopes; Human; Immunoglobulin G/analysis; Intermediate Filament Proteins/*immunology; Kuru/*immunology; Mice; Molecular Weight; Nervous System Diseases/*immunology; Species Specificity
SP englisch
PO USA