NR ALIU
AU Takahashi,H.; Iwata,T.; Kitagawa,Y.; Takahashi,R.H.; Sato,Y.; Wakabayashi,H.; Takashima,M.; Kido,H.; Nagashima,K.; Kenney,K.; Gibbs,C.J.Jr.; Kurata,T.
TI Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease
QU Clinical and Diagnostic Laboratory Immunology 1999 Nov; 6(6): 983-5
PT journal article
AB We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.
MH Antibodies, Monoclonal/diagnostic use; Antibody Specificity; Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid/*diagnosis; Human; Hybridomas; Immunoblotting; Isomerism; *Proteins/chemistry/immunology/metabolism; Support, Non-U.S. Gov't
AD Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
SP englisch
PO USA