NR ALGT
AU Supattapone,S.; Muramoto,T.; Legname,G.; Mehlhorn,I.; Cohen,F.E.; DeArmond,S.J.; Prusiner,S.B.; Scott,M.R.D.
TI Identification of two prion protein regions that modify scrapie incubation time
QU Journal of Virology 2001 Feb; 75(3): 1408-13
PT journal article
AB A series of prion transmission experiments was performed in transgenic (Tg) mice expressing either wild-type, chimeric, or truncated prion protein (PrP) molecules. Following inoculation with Rocky Mountain Laboratory (RML) murine prions, scrapie incubation times for Tg(MoPrP)4053, Tg(MHM2)294/Prnp(0/0), and Tg(MoPrP, Delta23-88)9949/Prnp(0/0) mice were approximately 50, 120, and 160 days, respectively. Similar scrapie incubation times were obtained after inoculation of these lines of Tg mice with either MHM2(MHM2(RML)) or MoPrP(Delta23-88)(RML) prions, excluding the possibility that sequence-dependent transmission barriers could account for the observed differences. Tg(MHM2)294/Prnp(0/0) mice displayed prolonged scrapie incubation times with four different strains of murine prions. These data provide evidence that the N terminus of MoPrP and the chimeric region of MHM2 PrP (residues 108 through 111) both influence the inherent efficiency of prion propagation.
MH Animal; Epitopes; Mice; Mice, Transgenic; Prions/chemistry/*physiology; Scrapie/*etiology; Species Specificity; Structure-Activity Relationship; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Time Factors
AD Institute for Neurodegenerative Diseases, University of California, San Francisco, California 94143, USA
SP englisch
PO USA