NR AKYH
AU Sklaviadis,T.K.; Manuelidis,L.; Manuelidis,E.E.
TI Physical properties of the Creutzfeldt-Jakob disease agent
QU Journal of Virology 1989 Mar; 63(3): 1212-22
PT journal article
AB In this report, we present the first physical characterization of the Creutzfeldt-Jakob disease agent. Preparations with high yields of infectivity (assayed infectious units) were obtained by a novel, gentle procedure in which initially sedimenting Gp34 ("prion" protein) was disaggregated by a variety of criteria with no subsequent loss of infectivity. Studies with this preparation indicate that most of the Creutzfeldt-Jakob disease agent has both a viruslike size and density. In velocity sedimentation and isopycnic sucrose gradients, infectivity comigrated with nucleic acid-protein complexes of appreciable size.
MH Animal; Brain/microbiology; Cell Fractionation/methods; Centrifugation, Density Gradient; Creutzfeldt-Jakob Syndrome/*microbiology; Hamsters; Hydrogen-Ion Concentration; Nucleoproteins/isolation & purification/*ultrastructure; Solubility; Support, U.S. Gov't, P.H.S.; Viruses, Unclassified/isolation & purification/*ultrastructure
AD Section of Neuropathology, Yale University School of Medicine, New Haven, Connecticut 06510.
SP englisch
PO USA