NR AKYE
AU Sklaviadis,T.K.; Dreyer,R.; Manuelidis,L.
TI Analysis of Creutzfeldt-Jakob disease infectious fractions by gel permeation chromatography and sedimentation field flow fractionation
QU Virus Research 1992 Dec; 26(3): 241-54
PT journal article
AB Gel permeation chromatography and sedimentation field flow fractionation (SF3) were used to further analyze highly infectious fractions from Creutzfeldt-Jakob disease (CJD) infected hamster brain. These analyses defined the relative molecular mass and physical size of the Creutzfeldt-Jakob disease (CJD) agent with greater precision than previously possible. Highly purified disaggregated fractions yielded single, homogeneous Gaussian peaks with both methods. The relevant analytical peaks contained protein-nucleic acid complexes with an M(r) of approximately 1.5 x 10(7) daltons and a mean radius of approximately 30 nm. The experimental evidence further solidifies the concept of an infectious agent that resembles a viral core rather than a simple protein.
MH Animal; Brain/*microbiology; Brain Chemistry; Centrifugation, Density Gradient; Chromatography, High Pressure Liquid; Creutzfeldt-Jakob Syndrome/*microbiology; Hamsters; Molecular Weight; Nucleic Acids/analysis; Prions/chemistry/*isolation & purification; Support, U.S. Gov't, P.H.S.
AD Yale University, School of Medicine, New Haven, CT 06510.
SP englisch
PO Niederlande