NR AKWX
AU Simonic,T.; Duga,S.; Strumbo,B.; Asselta,R.; Ceciliani,F.; Ronchi,S.
TI cDNA cloning of turtle prion protein
QU FEBS Letters 2000 Mar 3; 469(1): 33-8
PT journal article
AB Cloning of the cDNA coding for the 270-residue turtle prion protein is reported. It represents the most remote example thus far described. The entire coding region is comprised in a single exon, while a large intron interrupts the 5' UTR. The common structural features of the known prion proteins are all conserved in turtle PrP, whose identity degree to mammalian and avian proteins is about 40 and 58%, respectively. The most intriguing feature, unique to the turtle prion, is the presence of an EF-hand Ca(2+) binding motif in the C-terminal half of the protein.
MH Amino Acid Sequence; Animal; Base Sequence; Calcium-Binding Proteins/metabolism; Cloning, Molecular; Comparative Study; Conserved Sequence; Models, Molecular; Molecular Sequence Data; Prions/*genetics; RNA, Messenger/metabolism; Sequence Alignment; Support, Non-U.S. Gov't; Turtles/*genetics
AD Istituto di Fisiologia Veterinaria e Biochimica, Universita di Milano, Via Celoria 10, 20133, Milan, Italy. tatjana.simonic@unimi.it
SP englisch
PO Niederlande