NR AKWG
AU Silei,V.; Fabrizi,C.; Venturini,G.; Tagliavini,F.; Salmona,M.; Bugiani,O.; Lauro,G.M.
TI Measurement of intracellular calcium levels by the fluorescent Ca(2+) indicator Calcium-Green
QU Brain Research. Brain Research Protocols 2000 Apr; 5(2): 132-4
PT journal article
AB The fluorescent calcium-sensitive indicators, such as the Calcium Green-1, allow one to detect small calcium transients at low indicator concentrations. The protocol reported here is a rapid and sensitive method that facilitates the measurement of intracellular free-calcium in cell suspensions. Using this assay, we were able to detect and quantify the variations in intracellular calcium concentration during microglial cell activation induced by the fragment peptides beta25-35 and PrP106-126.
MH Amyloid beta-Protein/pharmacology; Calcium/*metabolism; Evaluation Studies; *Fluorescent Dyes; Human; Intracellular Membranes/*metabolism; Microglia/drug effects/metabolism/physiology; Osmolar Concentration; Peptide Fragments/pharmacology; Prions/pharmacology; Support, Non-U.S. Gov't
AD Dipartimento di Biologia, Universita di Roma Tre, Viale Marconi 446, 00146, Rome, Italy.
SP englisch
PO Niederlande