NR AKJH
AU Sanchez,A.; Guzman,A.; Ortiz,A.; Rembao,D.; Espinosa,B.; Zenteno,E.; Guevara,J.
TI Toluidine blue-O staining of prion protein deposits
QU Histochemistry and Cell Biology 2001 Dec; 116(6): 519-24
PT journal article
AB Prion diseases or transmissible spongiform encephalopathies are a group of fatal neurodegenerative diseases caused by an abnormal form of prion protein (PrPsc). In this study, we developed a sensitive histochemical detection of PrPsc deposits in a Gerstmann-Sträussler-Scheinker disease (GSS) patient using toluidine blue-O staining, a specific reagent to stain mucins and mucopolysaccharides. Detection of prion deposits correlated with immunohistochemistry using anti-prion antibodies. Control assays were performed using amyloid-beta (Abeta) plaques from Alzheimer's disease (AD) brains. Our results demonstrated that toluidine blue-O staining allowed to recognize 69.1+/-2.6% of the total plaques recognized by the anti-prion antibody. Furthermore, in the 15 studied brain regions from the GSS patient, toluidine blue-O revealed the same recognition pattern as anti-prion labeling. Toluidine blue-O stained specifically the prion deposits but not the Abeta plaques in AD brains. The specificity of the technique was confirmed in a Creutzfeldt-Jakob disease brain. This method opens several possibilities for postmortem diagnoses. Our results also suggest the relevance of specific post-translational modifications of PrPsc, identified by toluidine blue-O, that might participate in the transformation of PrPc to PrPsc.
MH Aged; Alzheimer Disease/metabolism/pathology; Amyloid beta-Protein/analysis/metabolism; Brain Chemistry; Creutzfeldt-Jakob Syndrome/metabolism/pathology; *Dyes; Fluorescent Antibody Technique, Indirect; Gerstmann-Sträussler-Scheinker Disease/metabolism/pathology; Human; Immunoenzyme Techniques; Middle Age; PrPsc Proteins/analysis/*metabolism; Sensitivity and Specificity; Staining and Labeling; Support, Non-U.S. Gov't; *Tolonium Chloride
AD Departamento de Neuropatologia, Instituto Nacional de Neurologia, Insurgentes Sur 3877, La Fama, 14269 Mexico, DF.
SP englisch
PO Deutschland