NR AJPQ
AU Priola,S.A.; Caughey,B.W.; Race,R.E.; Chesebro,B.
TI Heterologous PrP molecules interfere with accumulation of protease-resistant PrP in scrapie-infected murine neuroblastoma cells
QU Journal of Virology 1994 Aug; 68(8): 4873-8
PT journal article
AB Mutations within a host cellular protein, PrP, have been associated with disease in the transmissible spongiform encephalopathies. Murine neuroblastoma cells persistently infected with mouse scrapie accumulate protease-resistant PrP (PrPres), the abnormal form of PrP associated with disease in the transmissible spongiform encephalopathies. These cells provide a controlled system in which to study the molecular interactions which are important in the formation of PrPres. We have expressed recombinant PrP molecules in mouse scrapie-infected murine neuroblastoma cells and assayed the effect of these heterologous PrP genes on the formation and accumulation of PrPres. The results demonstrate that expression of heterologous PrP molecules which differ from the endogenous PrP by as little as one amino acid can profoundly interfere with the overall accumulation of PrPres. The data suggest that precise interactions between homologous PrP molecules are important in PrPres accumulation and that heterologous PrP molecules can block these interactions.
IN Andauernd mit Maus-Scrapie infizierte Mausneuroblastomzellen akkumulieren die proteaseresistente Form des Prionproteins. Diese Akkumulation kann stark reduiziert sein, wenn sich die beiden Prionprotein-Allele der Zellen auch nur um 1 Aminosäure unterscheiden.
MH Animal; Base Sequence; DNA, Viral; Endopeptidases/metabolism; Methionine/metabolism; Mice; Molecular Sequence Data; Neuroblastoma; PrPsc Proteins; Prions/*metabolism/pathogenicity; Recombinant Proteins/metabolism; Tumor Cells, Cultured; Virus Replication
AD Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840.
SP englisch
PO USA