NR AJNE
AU Pollack,S.J.; Sadler,I.I.J.; Hawtin,S.R.; Tailor,V.J.; Shearman,M.S.
TI Sulfated glycosaminoglycans and dyes attenuate the neurotoxic effects of beta-amyloid in rat PC12 cells
QU Neuroscience Letters 1995 Jan 23; 184(2): 113-6
PT journal article
AB Glycosaminoglycan (GAG)-containing proteoglycans are associated with the neuritic plaques and cerebrovascular beta-amyloid deposits of Alzheimer's disease as well as with the amyloid deposits of prion and other disorders. GAGs and other sulfate-containing compounds have previously been shown to bind beta-amyloid peptide in vitro, suggesting possible effects of beta-amyloid deposition and/or toxicity in vivo. Using reduction of the redox dye 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to measure beta-amyloid neurotoxicity in rat pheochromocytoma PC12 cells, several polysulfated GAGs and synthetic sulfate-containing compounds were found to attenuate the neurotoxic effects of beta-amyloid fragments beta 25-35 and beta 1-40. These results suggest that by binding beta-amyloid these compounds may prevent toxic interactions of the peptide with cells.
MH Amyloid beta-Protein/*antagonists & inhibitors/toxicity; Animal; Cell Survival/drug effects; Dyes/*pharmacology; Glycosaminoglycans/*pharmacology; Oxidation-Reduction; PC12 Cells; Peptide Fragments/antagonists & inhibitors/toxicity; Rats; Tetrazolium Salts/pharmacology; Thiazoles/pharmacology
AD Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK
SP englisch
PO Irland