NR AIZH
AU Oberdieck,U.; Xi,Y.G.; Pocchiari,M.; Diringer,H.
TI Characterisation of antisera raised against species-specific peptide sequences from scrapie-associated fibril protein and their application for post-mortem immunodiagnosis of spongiform encephalopathies
QU Archives of Virology 1994; 136(1-2): 99-110
PT journal article
AB Transmissible spongiform encephalopathies (TSE), such as scrapie or Creutzfeldt-Jakob disease (CJD), are fatal neurodegenerative diseases of the central nervous system caused by a yet unidentified virus. They are accompanied by a brain specific amyloidosis, during which a host coded protein irreversibly aggregates to form the scrapie-associated fibrils. The diagnosis of TSE relies on histopathological detection of spongiform lesions, on electron microscopical detection of fibrils, or on the immunological detection of SAF protein, which is the most specific diagnostic marker. In order to improve the diagnosis of TSE, we developed a protocol for rapid tissue fractionation and enrichment of SAF protein which subsequently allows the specific detection of SAF protein by western blotting and immunodetection. Using some new antisera raised against synthetic peptides with sequences specific for the hamster, sheep, cattle and human SAF protein, several samples can be diagnosed for TSE within 24 hours, starting with only 10-100 mg of brain tissue from different species.
IN Die Autoren Oberdieck, Xi, Pocchiari und Diringer behaupten immer noch, dass die übertragbaren spongiformen Enzephalopathien durch Viren übertragen würden. Für einen schnellen Nachweis der Krankheiten benutzen sie Antikörper gegen Prionproteine, die sie aber lieber SAF-Proteine nennen. Ausgehend von 10-100 mg Gehirnmasse wollen sie damit binnen 24 Stunden eine Diagnose stellen können. Es ist bestürzend, dass Prof. Diringer seine Position in diesem Artikel ohne die nötige Selbstkritik einfach als wahr darstellt.
MH Amino Acid Sequence; Animal; Antibodies, Viral/*immunology; Blotting, Western; Cattle; Hamsters; Human; Immune Sera; Immunoblotting; Immunologic Tests; Molecular Sequence Data; Nerve Tissue Proteins/*immunology; Peptide Fragments/immunology; Polymerase Chain Reaction; PrP 27-30 Protein; Prion Diseases/*diagnosis/immunology; Sheep; Species Specificity; Support, Non-U.S. Gov't
AD H. Diringer, Robert-Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, D-13353 Berlin, Federal Republic of Germany.
SP englisch
PO Österreich
OR Prion-Krankheiten 6