NR AIVL
AU Nemoto,T.; Horiuchi,M.; Ishiguro,N.; Shinagawa,M.
TI Detection methods of possible prion contaminants in collagen and gelatin
QU Archives of Virology 1999; 144(1): 177-84
PT journal article
AB We describe methods for the preparation of collagen and gelatin samples to detect possible prion contaminants using Western blotting of a major component of prions, PrPsc. A commercially available collagen solution containing 2% athero-collagen was spiked with rodent adapted scrapie prion and used as the prion-contaminating collagen. The methods developed center on the enzymatic reduction of the collagen solution viscosity with protease treatments and on the concentration of the prion from the protease-digests with polyethylene glycol-#6000 and NaCl. Recovery of the spiked prion as a partially protease-resistant core fragment of PrPsc fluctuated from 30% to 46% of the input amount.
MH Animal; Blotting, Western/methods; Collagen; *Drug Contamination; Gelatin; Prions/*isolation & purification; Support, Non-U.S. Gov't
AD Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
SP englisch
PO Österreich