NR AIDS
AU McBride,P.A.; Eikelenboom,P.; Kraal,G.; Fraser,H.; Bruce,M.E.
TI PrP protein is associated with follicular dendritic cells of spleens and lymph nodes in uninfected and scrapie-infected mice
QU Journal of Pathology 1992 Dec; 168(4): 413-8
PT journal article
AB Abnormal forms of a host protein, PrP, accumulate in the central nervous system in scrapie-affected animals. Here, PrP protein was detected immunocytochemically in tissue sections of spleen, lymph node, Peyer's patches, thymus, and pancreas from uninfected mice and from mice infected with a range of mouse-passaged scrapie strains and bovine spongiform encephalopathy (BSE). In the spleen, lymph node and Peyer's patches, PrP-positive cells were identified as follicular dendritic cells (FDC) by their location, appearance, and immune complex trapping function, whereas in the thymus they appeared to be two types of stromal cells: interdigitating cells (IDC) and cortical epithelial cells. In pancreas, PrP-containing cells were confined to the islets of Langerhans. Although the distribution of PrP immunolabelling was the same in tissues from scrapie-affected and uninfected mice, there was evidence that PrP accumulated in abnormal forms in FDC of infected mice. If, as is likely, PrP is essential for agent replication, our results suggest that FDC are the site of scrapie and BSE replication in the spleen and lymph node.
IN Das Mausprionprotein PrP wurde immunologisch in follikularen Zellen mit Dendriten in Milz und Lymphknoten, in Thymusstromazellen und in Langerhanszellen in Pankreasinseln nachgewiesen. Die Verteilung des Prionproteins war in normalen und in scrapieinfizierten Mäusen gleich. In scrapieinfizierten Mäusen scheint aber die veränderte Form des Prionproteins in Milz und Lymphknoten zu akkumulieren.
MH Animal; Cattle; Dendritic Cells/*chemistry; Encephalopathy, Bovine Spongiform/metabolism; Immunoenzyme Techniques; Lymph Nodes/*chemistry; Mice; Mice, Inbred Strains; Pancreas/chemistry; Peyer's Patches/chemistry; PrPsc Proteins; Prions/*analysis; Scrapie/*metabolism; Spleen/*chemistry
AD Patricia A. McBride (tricia.mcbride@bbsrc.ac.uk), Hugh Fraser (HughFraser@bbsrc.ac.uk), Moira E. Bruce (moira.bruce@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO England