NR AHYV
AU Masison,D.C.; Edskes,H.K.; Maddelein,M.L.; Taylor,K.L.; Wickner,R.B.
TI [URE3] and [PSI] are prions of yeast and evidence for new fungal prions
QU Current Issues in Molecular Biology 2000 Apr; 2(2): 51-9
PT journal article; review; review, tutorial
AB [URE3] and [PSI] are two non-Mendelian genetic elements discovered over 25 years ago and never assigned to a nucleic acid replicon. Their genetic properties led us to propose that they are prions, altered self-propagating forms of Ure2p and Sup35p, respectively, that cannot properly carry out the normal functions of these proteins. Ure2p is partially protease-resistant in [URE3] strains and Sup35p is aggregated specifically in [PSI] strains supporting this idea. Overexpression of Hsp104 cures [PSI], as does the absence of this protein, suggesting that the prion change of Sup35p in [PSI] strains is aggregation. Strains of [PSI], analogous to those described for scrapie, have now been described as well as an in vitro system for [PSI] propagation. Recently, two new potential prions have been described, one in yeast and the other in the filamentous fungus, Podospora.
ZR 80
MH Amino Acid Sequence; Comparative Study; Fungal Proteins/genetics; Genes, Fungal; Models, Genetic; Molecular Sequence Data; Mutation; Phenotype; PrPsc Proteins/genetics; Prions/*genetics; Saccharomyces cerevisiae/*genetics; Sordariales/genetics
AD National Institutes of Health, Bethesda, MD 20892-0830, USA
SP englisch
PO England