NR AHNG
AU Lopez,C.D.; Yost,C.S.; Prusiner,S.B.; Myers,R.M.; Lingappa,V.R.
TI Unusual topogenic sequence directs prion protein biogenesis
QU Science 1990 Apr 13; 248(4952): 226-9
PT journal article
AB Biosynthetic studies of the prion protein (PrP) have shown that two forms of different topology can be generated from the same pool of nascent chains in cell-free translation systems supplemented with microsomal membranes. A transmembrane form is the predominant product generated in wheat germ (WG) extracts, whereas a completely translocated (secretory) form is the major product synthesized in rabbit reticulocyte lysates (RRL). An unusual topogenic sequence within PrP is now shown to direct this system-dependent difference. The actions of this topogenic sequence were independent of on-going translation and could be conferred to heterologous proteins by the engineering of a discrete set of codons. System-dependent topology conferred by addition of RRL to WG translation products suggests that this sequence interacts with one or more cytosolic factors.
MH Animal; Codon; DNA, Viral/genetics; Hamsters; Kinetics; Mesocricetus; Peptide Mapping; Plasmids; PrPsc Proteins; Prions/*genetics; Protein Processing, Post-Translational; Restriction Mapping; Support, U.S. Gov't, P.H.S.; Transcription, Genetic; Translation, Genetic; Viral Proteins/biosynthesis/*genetics
AD Department of Physiology, University of California, San Francisco 94143.
SP englisch
PO USA