NR AHLW
AU Liu,H.; Farr-Jones,S.; Ulyanov,N.B.; Llinas,M.; Marqusee,S.; Groth,D.; Cohen,F.E.; Prusiner,S.B.; James,T.L.
TI Solution structure of Syrian hamster prion protein rPrP(90-231)
QU Biochemistry 1999 Apr 27; 38(17): 5362-77
PT journal article
AB NMR has been used to refine the structure of Syrian hamster (SHa) prion protein rPrP(90-231), which is commensurate with the infectious protease-resistant core of the scrapie prion protein PrPsc. The structure of rPrP(90-231), refolded to resemble the normal cellular isoform PrPc spectroscopically and immunologically, has been studied using multidimensional NMR; initial results were published [James et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 10086-10091]. We now report refinement with better definition revealing important structural and dynamic features which can be related to biological observations pertinent to prion diseases. Structure refinement was based on 2778 unambiguously assigned nuclear Overhauser effect (NOE) connectivities, 297 ambiguous NOE restraints, and 63 scalar coupling constants (3JHNHa). The structure is represented by an ensemble of 25 best-scoring structures from 100 structures calculated using ARIA/X-PLOR and further refined with restrained molecular dynamics using the AMBER 4.1 force field with an explicit shell of water molecules. The rPrP(90-231) structure features a core domain (residues 125-228), with a backbone atomic root-mean-square deviation (RMSD) of 0.67 A, consisting of three alpha-helices (residues 144-154, 172-193, and 200-227) and two short antiparallel beta-strands (residues 129-131 and 161-163). The N-terminus (residues 90-119) is largely unstructured despite some sparse and weak medium-range NOEs implying the existence of bends or turns. The transition region between the core domain and flexible N-terminus, i.e., residues 113-128, consists of hydrophobic residues or glycines and does not adopt any regular secondary structure in aqueous solution. There are about 30 medium- and long-range NOEs within this hydrophobic cluster, so it clearly manifests structure. Multiple discrete conformations are evident, implying the possible existence of one or more metastable states, which may feature in conversion of PrPc to PrPsc. To obtain a more comprehensive picture of rPrP(90-231), dynamics have been studied using amide hydrogen-deuterium exchange and 15N NMR relaxation times (T1 and T2) and 15N inverted question mark1H inverted question mark NOE measurements. Comparison of the structure with previous reports suggests sequence-dependent features that may be reflected in a species barrier to prion disease transmission.
ZR 78 Zitate
MH Amino Acid Sequence; Animal; Comparative Study; Hamsters; Mesocricetus; Models, Molecular; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Peptide Fragments/*chemistry/genetics; PrP 27-30 Protein/*chemistry/genetics; Prions/chemistry; Protein Conformation; Protein Structure, Secondary; Recombinant Proteins/*chemistry; Scrapie/metabolism; Solutions; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Thermodynamics
AD Department of Pharmaceutical Chemistry, University of California, San Francisco 94143, USA
SP englisch
PO USA
EA pdf-Datei und HTML-Version
OR Prion-Krankheiten L