NR AHCZ
AU Leclerc,E.; Liemann,S.; Wildegger,G.; Vetter,S.W.; Nilsson,F.
TI Selection and characterization of single chain Fv fragments against murine recombinant prion protein from a synthetic human antibody phage display library
QU Human Antibodies 2000; 9(4): 207-14
PT journal article
AB We describe the selection of single chain Fv fragments (scFv) against recombinant murine prion protein (mPrP) from a synthetic human antibody phage display library. Six different antibodies were isolated after three rounds of panning against full-length mPrP. All antibodies recognized a truncated form of mPrP containing residues (121-231). The amino acid sequence of the CDR3 of the scFv fragments has been determined. Five of the antibodies have been over-expressed, purified and their affinity for full-length mPrP determined by ELISA. The observed binding affinities vary from 30 nM to 2.7 microM.
MH Amino Acid Sequence/genetics; Animal; Complementarity Determining Regions/genetics/immunology/*isolation &; purification; Enzyme-Linked Immunosorbent Assay; Gene Library; Human; Immunoglobulin Fragments/*immunology/*isolation & purification; Mice; Prions/genetics/*immunology; Recombinant Proteins/genetics/*immunology; Support, Non-U.S. Gov't
AD Institute of Molecular Biology and Biophysics, ETH Hönggerberg, Zürich, Switzerland. estelle@scripps.edu
SP englisch
PO Niederlande