NR AGZA
AU Lahiff,S.; Glennon,M.; O'Brien,L.; Lyng,J.; Smith,T.; Maher,M.; Shilton,N.
TI Species-specific PCR for the identification of ovine, porcine and chicken species in meta and bone meal (MBM)
QU Molecular and Cellular Probes 2001 Feb; 15(1): 27-35
PT journal article
AB BSE, first identified in the UK in 1986 is thought to have arisen from feeding scrapie infected Meat and Bone Meal (MBM), produced under sub-optimal conditions, to cattle. For quality and safety reasons there is a requirement for a good analytical test for the surveillance of processed MBM. This study describes species-specific PCR assays for the identification of ovine, porcine and poultry species in MBM. A comparison between two distinct DNA extraction methods, i.e. the silicaguanidiumthiocyanate DNA isolation procedure and a commercial DNA extraction kit, is also presented. Application of this technology to species identification in industrial MBM was investigates as part of this study.
MH Animal; Animal Feed/*analysis; Biotechnology; Chickens; Comparative Study; DNA/*analysis; Endonucleases/metabolism; *Food Analysis; Guanidine/analysis; Meat/*analysis; Molecular Sequence Data; Polymerase Chain Reaction/*methods; Scrapie; Sheep; Silicon Dioxide/analysis; Species Specificity; Support, Non-U.S. Gov't; Swine; Thiocyanates/metabolism
AD The National Diagnostics Centre, BioResearch Ireland, National University, Galway, Ireland. sineadlahiff@ireland.com
SP englisch
PO England