NR AGPY
AU Kochneva-Pervukhova,N.V.; Chechenova,M.B.; Valouev,I.A.; Kushnirov,V.V.; Smirnov,V.N.; Ter-Avanesyan,M.D.
TI [Psi(+)] prion generation in yeast: characterization of the 'strain' difference.
QU Yeast 2001 Apr; 18(6): 489-97
PT journal article
AB The yeast cytoplasmically-inherited nonsense suppressor [PSI(+)] determinant is presumed to be a manifestation of the aggregated prion-like state of the Sup35 protein. Overexpression of the Sup35 protein induces generation of [PSI(+)] determinants with various suppressor efficiency and mitotic stabilities. Here, we demonstrate that the relative frequency of appearance of [PSI(+)] with different properties depends on the SUP35 allele used to induce their generation. The difference in properties of [PSI(+)] determinants was preserved after their transmission from one yeast strain to another. This difference correlated with variation in properties of the Sup35 protein. A novel type of prion instability was observed: some [PSI(+)] with weak suppressor efficiency could convert spontaneously into strong suppressor determinants.
MH Alleles; Blotting, Western; Cell Aggregation/genetics; Cell Cycle; Fungal Proteins/biosynthesis/*genetics; Gene Expression Regulation, Fungal/genetics; Phenotype; Plasmids; Prions/genetics/metabolism; Saccharomyces cerevisiae/*genetics/metabolism; Species Specificity; Support, Non-U.S. Gov't; Suppression, Genetic/*genetics
AD Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia.
SP englisch
PO England