NR AGLT
AU Kimura,K.M.; Yokoyama,T.; Haritani,M.; Narita,M.; Bellerby,P.J.; Smith,J.; Spencer,Y.I.
TI In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique
QU Journal of Veterinary Diagnostic Investigation 2002 May; 14(3): 255-7
PT evaluation studies; journal article
AB To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrPc) and abnormal (PrPsc) isoforms of the prion protein. In BSE- or scrapie-affected brains, the PrPc signal decreased, especially in those areas where the PrPsc signal was detected.
MH Animal; Brain/pathology; Cattle; Encephalopathy, Bovine Spongiform/*diagnosis/pathology; Immunohistochemistry/*methods; Prions/*analysis; Protein Isoforms/analysis; Scrapie/*diagnosis/pathology; Sensitivity and Specificity; Sheep; Support, Non-U.S. Gov't
AD National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
SP englisch
PO USA