NR AGHY
AU Kenward,N.; Hope,J.; Landon,M.; Mayer,R.J.
TI Expression of polyubiquitin and heat-shock protein 70 genes increases in the later stages of disease progression in scrapie-infected mouse brain
QU Journal of Neurochemistry 1994 May; 62(5): 1870-7
PT journal article
AB We have shown by northern analyses that the expression of the mouse polyubiquitin C gene is increased severalfold in the brains of mice infected with both the ME7 and 87V strains of scrapie. Expression of the polyubiquitin gene does not change significantly, compared with controls, until the later stages of disease progression when there is a 2.5-fold increase in ME7-infected brains and a 1.8-fold increase in 87V-infected brains. The patterns of changes of expression of the polyubiquitin genes in brains infected with the two strains of scrapie resemble those of accumulation of ubiquitin-conjugate-positive structures in the brain that are detected immunohistochemically. A similar increase in the expression of a heat-shock protein 70 gene also occurs.
IN Bei mit Scrapie infizierten Mäusen wurde in der späten Krankheitsphase eine starke Zunahme der Expression von Polyubiquitin C und dem Hitzeschockprotein 70 in den erkrankten Gehirnen gefunden.
MH Animal; Base Sequence; Biopolymers/*biosynthesis/genetics; Blotting, Northern; Brain/*metabolism/physiopathology; Comparative Study; DNA Primers; *Gene Expression Regulation; Heat-Shock Proteins/*biosynthesis/genetics; Mice; Molecular Sequence Data; Polymerase Chain Reaction/methods; Polyubiquitin; Prions/classification/pathogenicity; RNA/isolation & purification/metabolism; Scrapie/*metabolism; Support, Non-U.S. Gov't; Ubiquitins/*biosynthesis/genetics
AD Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, England.
SP englisch
PO USA