NR AGHU
AU Kenney,K.; Brechtel,C.; Takahashi,H.; Kurohara,K.; Anderson,P.; Gibbs,C.J.Jr.
TI An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients
QU Annals of Neurology 2000 Sep; 48(3): 395-8
PT journal article
AB The detection of 14-3-3 protein by Western immunoblot is a sensitive and specific cerebrospinal fluid marker of Creutzfeldt-Jakob disease (CJD). We developed a quantitative enzyme-linked immunosorbent assay (ELISA) that reliably detects 14-3-3 in cerebrospinal fluid. In a prospective study of 147 cerebrospinal fluid samples, the mean 14-3-3 concentration among pathologically confirmed CJD patients (28.0+/-20.6 ng/ml, n = 41) is significantly higher than the mean in the cerebrospinal fluid of those with other neurological disorders (3.1+/-2.9 ng/ ml, n = 84). At a cutoff value of 8.3 ng/ml, the ELISA has a sensitivity of 92.7% and a specificity of 97.6%. The 14-3-3 ELISA supports a diagnosis of CJD in patients who fulfill clinical criteria for possible CJD.
MH Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid; Enzyme Inhibitors/*analysis; Enzyme-Linked Immunosorbent Assay/*methods; Human; Prospective Studies; Proteins/*analysis; Reproducibility of Results; Sensitivity and Specificity
AD Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4122, USA
SP englisch
PO USA