NR AGFD
AU Kascsak,R.J.; Rubenstein,R.; Merz,P.A.; Carp,R.I.; Wisniewski,H.M.; Diringer,H.
TI Biochemical differences among scrapie-associated fibrils support the biological diversity of scrapie agents
QU Journal of General Virology 1985 Aug; 66(8): 1715-22
PT journal article
AB Scrapie-associated fibrils (SAF) were isolated and purified from animals infected with three different scrapie agents: ME7 and 139A in mice, and 263K in hamsters. Mouse ME7 and 139A SAF differed from hamster 263K SAF in morphology, sedimentation rate and protein composition. SAF from the three scrapie agents were distinguishable from each other by their sensitivity to proteinase K digestion. SAF copurified with infectivity in both the hamster and mouse systems. SAF appear to be a unique class of structures which are related but specific for each individual scrapie agent. These properties may correlate with the biological and pathological differences seen among these agents.
IN Die Monomere der Scrapie-assoziierten Fibrillen in mit den Scrapiestämmen ME7 und 139A infizierten Mäusen, sowie bei 263K-infizierten Hamstern unterschieden sich anhand ihrer Resistenz gegenüber Protease K. In Abhängigkeit vom Erregerstamm unterschieden sich auch die Formen und Sedimentationsraten der Scrapie-assoziierten Fibrillen.
MH Animal; Comparative Study; Endopeptidase K; Endopeptidases; Hamsters; Mice; Mice, Inbred C57BL; Microscopy, Electron; Prions/isolation & purification/*ultrastructure; Species Specificity; Support, U.S. Gov't, P.H.S.; Trypsin; Viral Proteins/*analysis
SP englisch
PO England