NR AFOD
AU Horiuchi,M.; Yamazaki,N.; Ikeda,T.; Ishiguro,N.; Shinagawa,M.
TI A cellular form of prion protein (PrPc) exists in many non-neuronal tissues of sheep
QU Journal of General Virology 1995 Oct; 76(10): 2583-7
PT journal article
AB A cellular form of the prion protein (PrPc) is thought to be a substrate for an abnormal isoform of th eprion protein (PrPsc) in scrapie. PrPc is abundant in tissues of the central nervous system, but little is known about the distribution of PrPc in non-neuronal tissues of sheep, the natural host of scrapie. This study investigated the tissue distribution of PrPc in sheep. Although PrPc was abundant in neuronal tissues, it was detected in non-neuronal tissues such as spleen, lymph node, lung, heart, kidney, skeletal muscle, uterus, adrenal gland, parotid gland, intestine, proventriculus, abomasum and mammary gland. Neither PrPc nor PrP mRNA was detected in the liver. The tissue distribution of PrPc appears to be inconsistent with the tissues which possess scrapie infectivity, suggesting that factor(s) specific to certain cell types may be required to support multiplication of the scrapie agent.
IN Das normale zelluläre Prionprotein wurde beim Schaf außer im Zentralnervensystem auch in Milz, Lymphknoten, Lunge, Herz, Nieren, Skelettmuskeln, Uterus, Nebennierendrüsen, Ohrspeicheldrüse, Dünndarm, Proventriculus, Abomasum und Brustdrüsen. In der Leber wurde weder das normale Prionprotein, noch seine mRNA gefunden.
ZR 19
MH Amino Acid Sequence; Animal; Cattle; Cell Extracts; Cell Membrane/chemistry; Mice; Molecular Sequence Data; Organ Specificity; Peptides/chemical synthesis/immunology; PrPc Proteins/*analysis; RNA, Messenger/analysis; Sheep/*metabolism; Support, Non-U.S. Gov't
AD Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
SP englisch
PO England
OR Prion-Krankheiten H